LEM-PCR: a method for determining relative transcript isoform proportions using real-time PCR without a standard curve
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چکیده
منابع مشابه
High-resolution semi-quantitative real-time PCR without the use of a standard curve.
The repeatability and sensitivity of a simple, adaptable, semi-quantitative, real-time RT-PCR assay was investigated. The assay can be easily and rapidly applied to quantitate relative levels of any gene product without using standards, provided that amplification conditions are specific for the PCR product of interest. Using the LightCycler real-time PCR machine, a serial 10-fold dilution seri...
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ژورنال
عنوان ژورنال: Genome
سال: 2010
ISSN: 0831-2796,1480-3321
DOI: 10.1139/g10-036